ex vivo nearinfrared fluorescence nirf imaging system  (Revvity)

Journal: npj Imaging

Article Title: Near-infrared fatty acid molecular probe for image-guided surgery of glioblastoma

doi: 10.1038/s44303-025-00077-z

Figure Lengend Snippet: a , b In- and ex vivo fluorescence accumulation of FA-ICG ( a ) compared to fluorescence accumulation of ICG ( b ) at 8 h after drug administration on IVIS® Spectrum. Higher accumulation of FA-ICG (compared to ICG) is demonstrated on the right side of the head and in the right (tumor-bearing) brain hemisphere. c , d Coronal thionine-stained brain sections demonstrate tumor presence in the right cerebral hemisphere in two different mouse sections (in dark violet). e NIR imaging demonstrates increased NIR fluorescence signal in the same brain slice on flatbed imaging ( c , e show same slice), tumor presence co-localizes with NIR signal f Limited NIR fluorescence signal is observed in the ICG administered mouse, albeit slightly more in the tumor than in surrounding brain as compared with thionine staining ( d ), d , f show same slice. The sample size per experimental group described is n = 3. Error bars report on standard deviation. Statistical analyses were performed using a one-way ANOVA test followed by the Bonferroni–Dunn method for multiple mean comparison. Statistical significance was set at p < 0.05 (*< 0.05, **<0.01, ***<0.001).

Article Snippet: After which, the brains were fixed in PFA 4% and embedded in sucrose, so that afterwards the brains could be cut in 50 μm slices for ex vivo NIR fluorescence imaging on the ODYSSEY M scanner (LI-COR) at 800 nm with high resolution (5 μm), followed by the same-slice (histo)pathological staining in thionine for tumor-fluorescence co-localization.

Techniques: Ex Vivo, Fluorescence, Staining, Imaging, Slice Preparation, Standard Deviation, Comparison

Journal: npj Imaging

Article Title: Near-infrared fatty acid molecular probe for image-guided surgery of glioblastoma

doi: 10.1038/s44303-025-00077-z

Figure Lengend Snippet: Intraoperative fluorescence imaging demonstrates accumulation of FA-ICG probe, left panel ( b , d , f , h ), when compared to ICG, right panel ( c , e , g , i ), on QUEST Spectrum® 2 image-guided surgery camera. a Schematic representation of the timeline of the experiment. b , c Transcutaneous fluorescence imaging demonstrates diffuse fluorescence signal in FA-ICG and ICG administered mice. d , e Transcranial fluorescence imaging demonstrates localized fluorescence signal (point or dot-shaped) in FA-ICG administered mouse ( d ) while more diffuse signal is observed in case of ICG ( e ). Notably, the brightfield image ( e ) shows more hemorrhage below the skull (and not clear tumor). f , g Higher intraparenchymal NIR signal (as shown in f and h ) is demonstrated in the right cerebral hemisphere for FA-ICG, while in the ICG administered mouse ( g and i ) signal is observed in the middle (and the hemorrhage below the skull disappears). h , i Ex vivo imaging of the brain demonstrates considerably higher signal in the FA-ICG administered mouse than in the ICG administered mouse. The sample size per experimental group described is n = 3.

Article Snippet: After which, the brains were fixed in PFA 4% and embedded in sucrose, so that afterwards the brains could be cut in 50 μm slices for ex vivo NIR fluorescence imaging on the ODYSSEY M scanner (LI-COR) at 800 nm with high resolution (5 μm), followed by the same-slice (histo)pathological staining in thionine for tumor-fluorescence co-localization.

Techniques: Fluorescence, Imaging, Ex Vivo

Journal: npj Imaging

Article Title: Near-infrared fatty acid molecular probe for image-guided surgery of glioblastoma

doi: 10.1038/s44303-025-00077-z

Figure Lengend Snippet: A dog with a symptomatic mastocytoma located in the left upper hind leg was operated under NIR surgical camera guidance (PerkinElmer™, Solaris). a Schematic representation of fluorescence-guided surgery 10 h after administration of FA-ICG probe . b Intraoperative widefield and NIR fluorescence overlay of mastocytoma tumor demonstrates transcutaneous fluorescence signal of tumor (with skin removed around tumor) at 10 h post i.v. injection of 0.3 mg/kg FA-ICG imaging reagent. c Left tumor margin is exposed, demonstrating enhanced fluorescence signal from the tumor. d Lower, contralateral, right tumor margin is exposed, demonstrating fluorescence signal contralaterally. e Wound bed after resection demonstrates minimal remaining fluorescence signal. f Ex vivo fluorescence imaging of excised tumor with intact skin. g Ex vivo fluorescence imaging of excised tumor with open wound. (Arrows indicate tumor). Sample size of companion dogs included is n = 2. Surgery was performed by veterinarian Dr. Arno Roos (Veterinair Verwijscentrum Gouda, the Netherlands).

Article Snippet: After which, the brains were fixed in PFA 4% and embedded in sucrose, so that afterwards the brains could be cut in 50 μm slices for ex vivo NIR fluorescence imaging on the ODYSSEY M scanner (LI-COR) at 800 nm with high resolution (5 μm), followed by the same-slice (histo)pathological staining in thionine for tumor-fluorescence co-localization.

Techniques: Fluorescence, Injection, Imaging, Ex Vivo